Isolation and characterization of a full-length molecular DNA clone of Ghanaian HIV type 1 intersubtype A/G recombinant CRF02_AG, which is replication competent in a restricted host range.
Identifieur interne : 001074 ( Main/Exploration ); précédent : 001073; suivant : 001075Isolation and characterization of a full-length molecular DNA clone of Ghanaian HIV type 1 intersubtype A/G recombinant CRF02_AG, which is replication competent in a restricted host range.
Auteurs : S. Kusagawa [Japon] ; Y. Takebe ; R. Yang ; K. Motomura ; W. Ampofo ; J. Brandful ; Y. Koyanagi ; N. Yamamoto ; T. Sata ; K. Ishikawa ; Y. Nagai ; M. TatsumiSource :
- AIDS research and human retroviruses [ 0889-2229 ] ; 2001.
Descripteurs français
- KwdFr :
- ADN viral, Adulte d'âge moyen, Cartographie nucléotidique, Données de séquences moléculaires, Femelle, Ghana, Humains, Phylogénie, Recombinaison génétique, Réaction de polymérisation en chaîne, Réplication virale, Séquence d'acides aminés, Séquence nucléotidique, Séropositivité VIH (virologie), VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (), VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (génétique).
- MESH :
- génétique : VIH-1 (Virus de l'Immunodéficience Humaine de type 1).
- virologie : Séropositivité VIH.
- ADN viral, Adulte d'âge moyen, Cartographie nucléotidique, Données de séquences moléculaires, Femelle, Ghana, Humains, Phylogénie, Recombinaison génétique, Réaction de polymérisation en chaîne, Réplication virale, Séquence d'acides aminés, Séquence nucléotidique, VIH-1 (Virus de l'Immunodéficience Humaine de type 1).
- Wicri :
- geographic : Ghana.
English descriptors
- KwdEn :
- MESH :
- chemical : DNA, Viral.
- geographic : Ghana.
- classification : HIV-1.
- genetics : HIV-1.
- virology : HIV Seropositivity.
- Amino Acid Sequence, Base Sequence, Female, Humans, Middle Aged, Molecular Sequence Data, Nucleotide Mapping, Phylogeny, Polymerase Chain Reaction, Recombination, Genetic, Virus Replication.
Abstract
We have isolated a replication-competent, full-length molecular clone of HIV-1 CRF02_AG, designated p97GH-AG1, by reconstituting two separately amplified genomic regions of an HIV-1 provirus of a 1997 Ghanaian isolate. The phylogenetic and recombination breakpoint analyses revealed that 97GH-AG1 had an A/G recombinant structure similar to that of prototype Nigerian isolate IbNG. The 17-nucleotide insertion downstream of the primer-binding site appeared to be a common sequence signature specific to most CRF02_AG strains, including 97GH-AG1. 97GH-AG1 showed an R5 phenotype and exerted productive infection in both HOS and NP2 cell infectivity assays, whereas it failed to show a detectable level of progeny production in peripheral blood mononuclear cells (PBMCs). The data may suggest the presence of unknown determinant(s) that dictate efficient replication in PBMCs, but that are not required for replication in immortalized cell lines.
DOI: 10.1089/088922201300119761
PubMed: 11375062
Affiliations:
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Le document en format XML
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Kusagawa</name><affiliation wicri:level="1"><nlm:affiliation>AIDS Research Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>AIDS Research Center, National Institute of Infectious Diseases, Tokyo 162-8640</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Takebe, Y" sort="Takebe, Y" uniqKey="Takebe Y" first="Y" last="Takebe">Y. Takebe</name></author><author><name sortKey="Yang, R" sort="Yang, R" uniqKey="Yang R" first="R" last="Yang">R. Yang</name></author><author><name sortKey="Motomura, K" sort="Motomura, K" uniqKey="Motomura K" first="K" last="Motomura">K. Motomura</name></author><author><name sortKey="Ampofo, W" sort="Ampofo, W" uniqKey="Ampofo W" first="W" last="Ampofo">W. Ampofo</name></author><author><name sortKey="Brandful, J" sort="Brandful, J" uniqKey="Brandful J" first="J" last="Brandful">J. Brandful</name></author><author><name sortKey="Koyanagi, Y" sort="Koyanagi, Y" uniqKey="Koyanagi Y" first="Y" last="Koyanagi">Y. Koyanagi</name></author><author><name sortKey="Yamamoto, N" sort="Yamamoto, N" uniqKey="Yamamoto N" first="N" last="Yamamoto">N. Yamamoto</name></author><author><name sortKey="Sata, T" sort="Sata, T" uniqKey="Sata T" first="T" last="Sata">T. Sata</name></author><author><name sortKey="Ishikawa, K" sort="Ishikawa, K" uniqKey="Ishikawa K" first="K" last="Ishikawa">K. Ishikawa</name></author><author><name sortKey="Nagai, Y" sort="Nagai, Y" uniqKey="Nagai Y" first="Y" last="Nagai">Y. Nagai</name></author><author><name sortKey="Tatsumi, M" sort="Tatsumi, M" uniqKey="Tatsumi M" first="M" last="Tatsumi">M. Tatsumi</name></author></analytic><series><title level="j">AIDS research and human retroviruses</title><idno type="ISSN">0889-2229</idno><imprint><date when="2001" type="published">2001</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>DNA, Viral</term><term>Female</term><term>Ghana</term><term>HIV Seropositivity (virology)</term><term>HIV-1 (classification)</term><term>HIV-1 (genetics)</term><term>Humans</term><term>Middle Aged</term><term>Molecular Sequence Data</term><term>Nucleotide Mapping</term><term>Phylogeny</term><term>Polymerase Chain Reaction</term><term>Recombination, Genetic</term><term>Virus Replication</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral</term><term>Adulte d'âge moyen</term><term>Cartographie nucléotidique</term><term>Données de séquences moléculaires</term><term>Femelle</term><term>Ghana</term><term>Humains</term><term>Phylogénie</term><term>Recombinaison génétique</term><term>Réaction de polymérisation en chaîne</term><term>Réplication virale</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Séropositivité VIH (virologie)</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1) ()</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (génétique)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA, Viral</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Ghana</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>HIV-1</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>HIV-1</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1)</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Séropositivité VIH</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>HIV Seropositivity</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Female</term><term>Humans</term><term>Middle Aged</term><term>Molecular Sequence Data</term><term>Nucleotide Mapping</term><term>Phylogeny</term><term>Polymerase Chain Reaction</term><term>Recombination, Genetic</term><term>Virus Replication</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN viral</term><term>Adulte d'âge moyen</term><term>Cartographie nucléotidique</term><term>Données de séquences moléculaires</term><term>Femelle</term><term>Ghana</term><term>Humains</term><term>Phylogénie</term><term>Recombinaison génétique</term><term>Réaction de polymérisation en chaîne</term><term>Réplication virale</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1)</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Ghana</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have isolated a replication-competent, full-length molecular clone of HIV-1 CRF02_AG, designated p97GH-AG1, by reconstituting two separately amplified genomic regions of an HIV-1 provirus of a 1997 Ghanaian isolate. The phylogenetic and recombination breakpoint analyses revealed that 97GH-AG1 had an A/G recombinant structure similar to that of prototype Nigerian isolate IbNG. The 17-nucleotide insertion downstream of the primer-binding site appeared to be a common sequence signature specific to most CRF02_AG strains, including 97GH-AG1. 97GH-AG1 showed an R5 phenotype and exerted productive infection in both HOS and NP2 cell infectivity assays, whereas it failed to show a detectable level of progeny production in peripheral blood mononuclear cells (PBMCs). The data may suggest the presence of unknown determinant(s) that dictate efficient replication in PBMCs, but that are not required for replication in immortalized cell lines.</div></front></TEI><affiliations><list><country><li>Japon</li></country><region><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement></list><tree><noCountry><name sortKey="Ampofo, W" sort="Ampofo, W" uniqKey="Ampofo W" first="W" last="Ampofo">W. Ampofo</name><name sortKey="Brandful, J" sort="Brandful, J" uniqKey="Brandful J" first="J" last="Brandful">J. Brandful</name><name sortKey="Ishikawa, K" sort="Ishikawa, K" uniqKey="Ishikawa K" first="K" last="Ishikawa">K. Ishikawa</name><name sortKey="Koyanagi, Y" sort="Koyanagi, Y" uniqKey="Koyanagi Y" first="Y" last="Koyanagi">Y. Koyanagi</name><name sortKey="Motomura, K" sort="Motomura, K" uniqKey="Motomura K" first="K" last="Motomura">K. Motomura</name><name sortKey="Nagai, Y" sort="Nagai, Y" uniqKey="Nagai Y" first="Y" last="Nagai">Y. Nagai</name><name sortKey="Sata, T" sort="Sata, T" uniqKey="Sata T" first="T" last="Sata">T. Sata</name><name sortKey="Takebe, Y" sort="Takebe, Y" uniqKey="Takebe Y" first="Y" last="Takebe">Y. Takebe</name><name sortKey="Tatsumi, M" sort="Tatsumi, M" uniqKey="Tatsumi M" first="M" last="Tatsumi">M. Tatsumi</name><name sortKey="Yamamoto, N" sort="Yamamoto, N" uniqKey="Yamamoto N" first="N" last="Yamamoto">N. Yamamoto</name><name sortKey="Yang, R" sort="Yang, R" uniqKey="Yang R" first="R" last="Yang">R. Yang</name></noCountry><country name="Japon"><region name="Région de Kantō"><name sortKey="Kusagawa, S" sort="Kusagawa, S" uniqKey="Kusagawa S" first="S" last="Kusagawa">S. Kusagawa</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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